Information on the depth at which plankton communities are sampled is necessary to study their ecology. When a plankton net is "towed vertically," if the net can be lowered straight down to the target depth (with the wire rope entering the water at right angles to the water surface), the net depth is the length of the wire rope unrolled from the winch. On the other hand, a vessel is always subject to wind and currents, so it cannot maintain its position. Also, the net itself is similarly affected by currents in the water, so it is possible for the net to move away from directly under the vessel. When the ship and the net are separated and the wire rope is tilted, the length of the unrolled wire rope is no longer equal to the net depth. Therefore, assuming that the wire rope is straight (not sagging) in the sea, the net depth can be obtained by adding up the sine (sinθ) of the length of the unrolled wire rope and the tilt angle (θ) shown in Figure 4. If the tilt angle (θ) is known, the wire length required to reach the desired target depth can also be determined. The instrument used to measure the inclination of the wire rope is called a tilt angle plate (Figure 5-a). The tilt angle plate consists of a semicircular protractor-like surface and a weighted pointer that points in a vertical downward direction. Care must be taken to tilt the tilt angle plate so that the pointer points vertically downward (Figure 5-c).