The amount of enzyme and reaction time were examined as conditions for degradation of xylan. After a certain reaction time, the reaction was subjected to thin-layer chromatography, and the concentration of the detected spots was used to determine what size oligosaccharides were being produced. We then determined the conditions under which the amount of monosaccharide produced was reduced and the amount of oligosaccharide produced was increased.
Figure 3. xylose and xylan amounts under different conditions with the two enzymes acting alon
e on dulse xylan.
