Summary

In "Development of a New Oligosaccharide Preparation Method from Red Algae, a Low-Use Resource," we introduced a method for preparing xylooligosaccharides, which have a structure unique to dulse. The preparation method used commercially available enzymes, which include two types of enzymes: endo-type enzymes that hydrolyze the polysaccharides from the inside and exo-type enzymes that hydrolyze the polysaccharides from the ends. Exo-type enzymes turn oligosaccharides into the monosaccharide xylose, thus reducing oligosaccharide yields. In this course, we prepared only the endo-type enzyme by acquiring the gene for the endo-type enzyme from microorganisms and having E. coli produce the enzyme based on the genetic information. We used it to prepare oligosaccharides.