The fertilization rate is calculated by observing the artificially inseminated eggs under a microscope and excluding those that do not from egg membrane or start cleavage.
Fertilization rate (%) = number of eggs fertilized normally / number of eggs artificially inseminated x 100
In addition, the hatching rate is calculated by counting the number of larvae that have hatched among the fertilized eggs incubated under the same conditions.
Hatching rate (%) = number of larvae hatched / number of fertilized eggs x 100
Fertilization rate, hatching rate, and survival rate of hatched larvae are the criteria to determine whether the egg quality was good or bad.