Eels would complete egg maturation and ovulation approximately 18 hours after DHP injection, and ready to spawn eggs.
The eggs taken from female are covered with sperm and placed in seawater for fertilization.
It is empirically known that fertilized eggs in good condition float. The floating rate can be calculated by counting the number of floating eggs out of all eggs. Floating rate (%) = number of floating eggs / number of artificially inseminated eggs x 100