토픽 개요

  • Tips for using pipettes correctly


     Pipettors (pipettes that use disposable tips to measure liquids with a dial adjustment) are useful. What must be followed was explained in one previous course. On top of that, we will explain the tips necessary to perform the experiment correctly.


    Points are,

     (1) Do not contaminate the reagents and instruments used in the experiment.

     (2) Ensure that the correct volume is aspirated and placed in the desired container.

    The key points are as follows.



    It is important that the tip of the pipette tip be below the liquid level, but it is better not to insert it unnecessarily too deeply.

    In particular, if the tip end contacts the bottom of the container, it may not be able to suck the liquid. It is natural.



    より正確に扱うために、To handle more accurately,

    不必要に先端を液面深くまで入れないDo not insert the tip unnecessarily deep into the liquid.

    チューブ外壁に付着する液が増えるMore liquid adheres to the outer wall of the tube

    先端が底面に密着して、吸えないThe tip adheres to the bottom and cannot be sucked




     To avoid contaminating the outside of the reagent bottle or pipette, lower it vertically and raise it vertically without making the slightest movement.

     It is good if you feel like a pipette craftsman and can operate it like a machine yourself.



    ピペットを垂直にボトル口に入れるPipette vertically into the bottle mouth

    ピペットをボトルに接触させないDo not allow the pipette to contact the bottle

    汚染を招くcause pollution 機械のように正確にPrecise as a machine. もしくは、ピペット操作を想定して予め広口ボトルを使うなど対策をOr, use a wide-mouth bottle in advance in anticipation of pipette operation.