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  • 一般

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  • How to use a pipette (pipettor) (procedure)

    • A pipette is a volumetric device for quantitative aspiration of small amounts of liquid.

      A pipettor is a tool that adjusts a dial to suck out any desired volume. It is very convenient and can suck out precisely.

      However, please observe the "must follow" and "must not do" rules. Not only will you fail to weigh, you will also damage your expensive pipettor.




    • Basic Usage


      　As shown in the picture below, when the piston is released from the depressed position, liquid is sucked out of the tip of the pipette. If the piston is pushed in again, the liquid can be produced.

      ★　Top" is the state in which the piston is released.

      ★　When the piston is pushed to the "1st stop" (first left in the picture below), it is in the "ready to suck out a certain volume" state.

      ★　When the piston is raised to Top (second left in the picture below) while sucking up liquid, it has "finished sucking up a certain volume.

      ★　When the piston is pushed to the "1st stop" (the third left in the picture below), it is in the "mostly discharged" state.

      ★　When the piston is pushed to the "2nd stop" (4th left in the picture below), it is in the state of "all discharged with the last push".

      ★　The piston was released and returned to its original state.





  • What not to do in pipette manipulation


    • Never put liquid into the body of a pipette.


      It boils down to this. In various situations, liquids are sucked up to the body, so we will note the precautions that must be taken.




    • Inhale slowly, exhale slowly, and release slowly while holding your fingers.

      Be sure to observe this.

      Failure to observe this will almost certainly cause the pipette to fail by sucking liquid up into the body.

      If you cannot follow this, do not touch the pipette. They are unsuitable for experiments.



      The following is an explanation of why and in what situations it is essential to "inhale slowly, dispense slowly, and release slowly with fingers attached".

      必ず守ることBe sure to observe

      ゆっくり液を吸い上げる、ゆっくり吐出して、ゆっくり解放するSuck up liquid slowly, exhale slowly, release slowly.

      ゆっくり（指を添えて）液を吸い上げるSlowly (with fingers) suck up the liquid

      液面の上昇を待つ。Wait for the liquid level to rise.

      （粘性の高い液体は上昇に数秒かかる）(Viscous liquids take a few seconds to rise)

      濃硫酸などConcentrated sulfuric acid, etc.

      ①　If the liquid is sucked up too vigorously, the momentum may cause the liquid to enter the body.

      ②　The liquid level rises slowly when a pipette is sucked up, such as highly viscous concentrated sulfuric acid. When the inside of a pipette is depressurized, but the pipette tip is taken out of the liquid surface, air may be sucked in with great force. The momentum of sucked air may cause the liquid to enter the body.



      液面が下がるthe liquid level drops　空気を吸い込むbreathe in air　勢いで液が跳ねるLiquid bounces with vigor.　本体に入り故障Failure to enter the main unit　先端が液面下にあることを常に確認Always make sure the tip is below the liquid level　常にゆっくり吸い上げることAlways inhale slowly.　ボトルの中が見えなくても確実にEven if you can't see what's in the bottle, you can be sure

      ★　As a matter of course, the liquid level in the container will drop if the liquid is sucked out with a pipette.If the pipette tip is at the very edge of the liquid level, air will be sucked in as the liquid level drops. So, the liquid will enter the body. Make sure the pipette tip is below the liquid level.

      ★　Some containers have no visible liquid level. Still, make sure that you do not inhale air.



      ゆっくり吐出する、ゆっくり（指を添えて）解放Slowly discharging, slowly (with fingers) releasing　早すぎる吐出Premature discharge　最後のワンプッシュOne last push　残ってるRemaining.　勢いよく開放Vigorously open　ゆっくり吐出Slow discharge　ゆっくり解放Slow Release

      ③　Reason for slow dispensing: When dispensing in a hurry, dispensing is completed even though there is still some liquid on the inner wall of the pipette tip. After the dispensing is completed, the liquid will run down the inner wall and accumulate at the tip of the pipette. Besides, if you release the pipette piston, you will let the liquid into the pipette body along with the air. Spit it out slowly and give it one last push (up to the 2nd stop) after it has passed through the inner wall and finished accumulating at the bottom. Pipette tips should be checked with a good visual inspection.

      　Reason for slowly releasing the tip with a finger: Even if liquid accumulates on the tip after dispensing is complete, it is often safe as long as the tip is slowly released with a finger.


      ※Finally, pushing and releasing the lever of the pipette and shpo shpo shpo is absolutely not allowed.



    • In addition to the "slow" rule, there is another important rule.


      Do not point the pipette tip upward


      A pipette before use can point upward, but do not point a pipette (tip) upward during or after use. If there is any liquid left, liquid will enter the body.

      Make it a habit not to point the pipette tip upward.


      必ず守ることBe sure to observe　ピペットの先端を上に向けないDo not point the pipette tip up.　内壁の液滴が本体に入り故障要因になるDroplets from the inner wall can enter the main unit and cause failure.　ピペットを持ちながらボトルの蓋を開けるときに注意Be careful when opening the bottle lid while holding the pipette.　指finger　このとき素人は、先端を上に向けてしまうAt this point, the layman turns the tip up.　先端を下に向けたまま、蓋を開けようOpen the lid with the tip pointing down.

      When performing operations such as opening the lid of a bottle while holding a pipette in one hand,

      If you are not used to this, you will turn the pipette tip upward (top of the right picture). Keep the pipette tip pointing down as shown in the lower right picture.