北海道大学FSC令和元年度公開水産科学実習：Hokkaido University, FSC Open Fisheries Science Training, 2018
応用発生工学実習：Applied Development Engineering Practice
亜寒帯魚類を対象とした増養殖実習：Aquaculture practice for subarctic fishes
Purpose of Practice
The observation in this practice begins with "finding what is instructed."But it shouldn't end on its own. Practical training aims at "cultivating the ability to observe" how the organization you are seeing is connected to others and how it changes.
Many of the experiments carried out in this training are special, but they are only technology, and become obsolete over time. However, I want you to think that the process in which you think and devise while using technology is "the greatest result of participating in the practical training." Reporting issues are prepared on such themes.
Details of practical training
(1) Observation of normal development
In order to manipulate the embryo for biological modifications, it is necessary to understand what morphogenetic processes the fertilized egg undergoes to produce tissues and organs. To do so, we first dissect the parental individuals, understand their structure, and observe the developmental process of artificially inseminated eggs.
(2) Chromosome manipulation in salmonids
Representative salmon fish as cultured fish have undergone biological modification using chromosome manipulation, such as sterilization and the creation of unisexual populations. Here, the following are carried out: Genetic inactivation by the ultraviolet irradiation to the sperm, half-body induction by the fertilization of the genetic inactivation sperm, female generation diploid by the second pole body emission inhibition, and induction experiment of the triploid. In addition, the induced hemiploid, diploid, and triploid are used to confirm polyploidy by FCM and chromosome specimens.
(3) Manipulation of salmon and carp fish embryos
Actually create manipulation instruments for manipulating fish embryos yourself. They are used for embryo manipulation, including ① excision of egg yolk, excision and transplantation of the blastocyst, ② injection of a solution into a fertilized egg or embryo, and ③ transplantation of embryonic cells. The development of embryos after these manipulations is observed, and the mechanism of fish development is observed and inferred.
(4) Conservation of genetic resources
Sperm cryopreservation will be conducted as a method of preserving genetic resources. The students will also experience artificial insemination using this frozen sperm.
Lectures related to each practice are given.
Professor Seiyano of Nagasaki University is also scheduled to give lectures and conduct experiments (with increased aquaculture in the southern part of Japan).
The challenge of the report is to write the know-how obtained by themselves according to the protocol of each experiment, and to describe how he or she has tackled the problem prepared.
Life during the practical training period
Accommodation facilities use the Usujiri Fisheries Laboratory. For accommodation guidance, read the instructions provided in each room and take appropriate action. Accommodation rooms are used jointly, so they should be used cleanly considering beds, bedding, shower rooms, etc. and people who use them later. On the last day, remove the sheets and covers and bring them to the prescribed location in the entrance.