Peptide sequences can be predicted from a mass spectrometer (results can be seen from the linked Table1).
We were able to find 42 peptides. Also, as shown in Figure 1(A), the major protein is phycobiliprotein. To find out if they contain peptide sequences, we examined the protein sequences using next-generation sequencing and polymerase chain reaction (PCR). Table 1 compares the sequences of phycoerythrin (LC599086), phycocyanin (LC599087), and allophycocyanin (LC599088), which are the phycobiliprotein components of upppleinori, with those of other red algae.
As a result, it showed high identity with laver (Pyropia pulchra, Porphyra purpurea, Pyropia yezoensis), and slightly lower identity with dulse (Devaleraea inkyuleei) and centipede laver (Grateloupia asiatica).
Table 1. Identity of the phycobiliproteins of Woodpecker and other red algae
