The hydrolyzate contains many peptides. In order to investigate which of these peptides has ACE inhibitory activity, we separated the hydrolyzate by high performance liquid chromatography (HPLC).
Figure 2(A) is the result of separation by HPLC. The numbered peaks in the figure were collected and examined for their ACE inhibitory activity. Figure 2(B) shows the ACE inhibitory activity of each fraction. It can be seen that the strength of activity differs for each fraction. Therefore, we examined what peptides were contained in each fraction using a mass spectrometer.
Figure 2. Separation of hydrolyzate and ACE inhibitory activity by high-performance liquid chromatography. (A) chromatograms of hydrolysates; (B) ACE inhibitory activities with numbers indicated in the chromatograms.
