Preparation of seaweed proteins and peptides

　As with other red algae, Wuppleinori protein is extracted from seaweed powder with water and dialyzed to remove low-molecular-weight components such as salts (preparation method link). Peptides derived from WuppuleInori protein used thermolysin, a protease.

Fig. 1(A) is the result of polyacrylamide electrophoresis (SDS-PAGE). Lane 2 shows protein bands. Lane 3 is the protein hydrolyzed with protease and has no band. Lanes 4 and 5 are fluorescence detection. In the protein lane there is phycobiliprotein fluorescence, while in the hydrolyzate there is fluorescence underneath the migration front.

　Figure 1(B) shows the ACE inhibitory activity. Proteins also have ACE inhibitory activity, but it was found that their hydrolysates greatly increased the inhibitory activity.

Figure 1. Characteristics of proteins and peptides derived from Wupppleinori

(A) SDS-PAGE. 1. marker protein; 2. Wupppleinori protein; 3. Wupppleinori peptide; 4. fluorescence detection of Wupppleinori protein; 5. fluorescence detection of Wupppleinori peptide

(B) Measurement of ACE inhibitory activity. *p < 0.05.