Sample preparation
1. Transfer a small piece of sample (just a little better) to a 1.5 ml test tube from. When the sample volume is large, the peak becomes difficult to understand at the time of measurement.
2. Place 150 μl of the pre-formulated solution A (stored under refrigeration) in the tube and vortex. Fluid A serves to make cells loose.
3. Vortex for 30 minutes.
4. Turn on the flow cytometer. To turn on the power, first turn on ① the breaker of the transformer, ② the printer (far right), and after 30 minutes, first turn on the pushbutton switch on the upper side of the switch on the rear side of the main body. Then, turn on the rocker switch (a switch that moves like a seesaw) at the bottom of the push-button switch. The reverse is true when it is deleted.
5. Fill in the lighting time of the fluorescent lamp.
6. Add 600-800 μl of solution B (DAPI) to the tube containing the sample and continue vortexing. DAPI: DNA-fluorescent stain
7. Fill the sample from above the tube with the mesh filter and drop it through the mesh. Add 200 μl of Solution B from the top of the mesh, add 200 μl of DW, and vortex thoroughly. Pressure may be applied from above the rubber cap filter prior to vortexing. (Go down, especially without applying pressure)
8. Press the CLEAN button on the flow cytometer to clean the flow path.
9. Wear the sample (the first control) and push the measurement in STOP→START order.
10. When a wavelength appears on the screen, adjust the peak of the wave to the value of 100. Press the GAINOOCUP or DOWN key to adjust (roughly 485 is exactly right) →ENTER or CLEAR.
11. If there is too much sample, press the Adjust →ENTER with SPEEDOOCUP or DOWN. If the peak is broad (the width of the bottom side is wide), dilute with solution B +DW=1:1.
12. Print the measurement results. STOP→FUNC→PRINT
13. Remove the sample and press the CLEAN to clean the flow path. The sample inlet is immersed in the sample, so the sample is washed by applying DW.
14. Samples are measured (repeats of 9, 11-13). If you want to compare multiple samples together, mix the samples. When the sample solution is low, add solution B and DW.
15. Turn off the power when the measurement is finished.
16. Write down the usage time of the fluorescent lamp in the usage book.
17. Cleaning the mesh: Let it soak in water and remove any dirt from the water stream. Clean with DW.
