① Female developmental haploid
1. Turn on the ultraviolet lamp.
2. Prepare a flat petri dish that has been cleaned and coated with Vaseline on the bottom sides.
3. Spermatozoa are diluted 50-100-fold with cherry trout spermatozoa (salmon) or artificial seminal plasma (carp fish).
4. Spread 0.5 ml of diluted spermatozoa of salmon trout or 100-200 μl of carp fish spermatozoa on the bottom of a flat petri dish to avoid air bubbles.
5. Process for 90 seconds with an ultraviolet lamp after 10 minutes or more after lighting.
6. UV-treated sperm (UV sperm) should be transferred to test tubes or hematocrit tubes and stored in a dry manner. (to avoid contamination with normal sperm)
7. Ultraviolet spermatozoa are inseminated into unfertilized eggs and fertilized by insemination (or fertilization).
② Male developmental haploid
1. Prepare a Petri dish with the saran wrap pinned and place a circular frame on the Petri dish.
2. Place the cherry ovarian cavity fluid in a circular frame so that unfertilized zebrafish or goldfish eggs are not attached to the edges.
3. Suck out the cherry salmon ovarian cavity fluid with a pipette man to the very edge of the eggs.
4. Place the petri dish in a box of ultraviolet lamps and treat with ultraviolet light (about 3 minutes).
5. After ultraviolet treatment, the ovarian cavity fluid is sucked out and sperm with normal sperm.
The fertilized egg is placed in the fertilized liquid and fertilized.
