① Prepare a needle for cell transplantation.
② Attach to the injector holder without putting anything in. Ensure that the mounting area is filled with mineral oil from the injector.
③ Rotate the injector knob clockwise to insert the mineral oil toward the needle tip and stop in front of the stenosis.
④ Draw the implant needle into the primary culture of the Petri dish used for implantation.
⑤ Turn the screw at the joint between the holder and the implant needle clockwise (in the closing direction), and then turn the needle tip of the implant needle upward.
⑥ Move the injector knob counter-clockwise to draw the primary culture into the implant needle.
⑦ Inhale and stop the primary culture to the middle of the implant needle.
⑧ Select the unfertilized egg and place it in the transplantation Petri dish, and insert the transplantation needle into the yolk portion.
⑨ Rotate the injector knob counter-clockwise and place the egg yolk in the needle (to the stenotic area) and coat the interior with egg yolk.
⑩ The primary culture is placed in and out of the implantation needle to wash out excess egg yolk. Remove unfertilized eggs.
⑪ Cell-labeled embryos (donor) and unlabeled embryos (host) are placed in transplanted Petri dishes.
⑫ Insert the implant needle into the labeled embryo's blastoderm. If it is inserted too deeply, YSL will be sucked in.
⑬ Turn the injector knob counterclockwise (slightly touching position) and draw deep cells (DC) into the implant needle. It is good that DC stops at the stenosis.
⑭ Pull the needle out of the donor embryo and insert it into the area of the host embryo to be implanted.
⑮ Turn the injector knob clockwise (slightly touching it) and slowly insert the DC in the implant needle into the host embryo.
⑯ The transplanted chimeric embryos are separated so that they do not mix with other embryos.
