① Ringer's solution for freshwater fish: 7. 5g NaCl, 0. 2g KCl, 0. 4g CaCl2/1 l DW
② Egg White: Take the egg white from the chicken egg, make a merenge, and store it in a refrigerator. Allow to stand for a while and use the egg white fluid accumulated in the lower part. Necessary reconciliation.
③ Primary culture: Ringer's solution for freshwater fish containing 1.6% egg white
④ Primary culture solution for 96-well culture dishes: Add 1/1000 volume of antibiotic to the solution in Ringer's solution for freshwater fish containing 1.6% egg white (antibiotic solution stock: penicillin, streptomycin are dissolved in DW at 10% concentration each, and dispense 100-200 μl into test tubes to keep them frozen)
⑤ Secondary culture solution: 1. 8 mM CaCl2, 1. 8 mM MgCl2 (180 μl of 1M CaCl2, and 180 μl of 1M MgCl2 are placed in 100ml of distilled water)
⑥ Agarose Petri dish already described
