① Put 150ml of Ringer for freshwater fish in a 300 ml beaker and put stirrer stirrer.
② Pour agar (Agar not for electrophoresis) to a concentration of 0.8-1%.
③ Cover with a saran wrap, put it on a stirrer, and stir the agar powder.
④ Put the Ringer's gel in a microwave oven and warm it up so that it does not boil suddenly.
⑤ Once warmed, place in a stirrer and stir the agar flour.
⑥ Repeat ③-⑤ to dissolve the agar, keeping the solution in it from boiling. When it becomes clear, put it over the light and check that no agar powder remains.
⑦ When the agar powder is completely dissolved, cool the agar solution a little.
⑧ During this time, the glass Petri dishes are placed on a desk (checking that they are level with a level gauge).
⑨ Pour the agar liquid into a glass petri dish. Thinner for embryo manipulation, but thicker agar for photographic purposes.
⑩ When the agar solidifies, cover, invert, and store in the refrigerator.。
