① Put DW into the syringe of the micro grinder and drip water onto the grinding wheel.
② Switch on the micro grinder so that the whole grinding wheel gets wet with DW.
③ Simply set the extended needle in the holder of the micro grinder.
④ Lower the holder angle to 30 degrees.
⑤ Operate the knob on the micro grinder to lower the needle to the surface of the grinder.
⑥ Operate the lens knob on the micro grinder to focus the pipette.
⑦ Rotate the coarse lever of the needle of the microgrinder and lower the tip of the pipette just before the surface of the grinding wheel.
⑧ Rotate the micro-motion lever to attach the needle tip to the grinding wheel surface.
⑨ Lower the needle with the micro-motion lever and start sharpening the tip.
⑩ When the mouth of the tip is empty about 10μm, turn the roughing lever to raise the whole needle and remove the needle.
⑪ Attach the needle to the dramont capillary pipette.
⑫ Put the tip in distilled water, take in and out distilled water, and wash off the grinding wheel powder.
⑬ Once, transfer to a needle case to dry.
⑭ As with microinjection needles, a stenosis is formed at the tip. For implantation, narrow the stenosis as much as possible. Note that if it is too thin, it will become clogged.
