① Flush both ends of the 10 Drummont glass capillaries with a gas burner to smooth the cut. Make sure that the mouth is not blocked because it is too hot.
② Place the glass capillary tube in a micropipetting machine and switch on and stretch the heater.
③ Remove the top of both the upper and lower glass needles so as not to break them, and place them in the injection needle case. Make 20 needles from 10 capillaries.
④ Set each needle on a micro-forge.
⑤ Operate the manipulator while looking at the microscope so that the tip of the glazing passes through the center of the microheater and enters the glass pillow.
⑥ The micro heater is energized while observing the microscope, and the stenosis is produced in the middle of the glass needle. For infusion, the stenosis should not be narrowed too much.
⑦ Turn off the power to the micro heater, and then move the manipulator to remove the tip of the needle from the micro heater.
⑧ Place the microinjection needle that forms the stenosis in the case.
