① A suitable thickness portion of the Pasteur pipette is required for the depression in the ampoule cutter to rotate the pipette.
② Check the pipette for cracks.
③ Hold both sides of the crack separately with the left and right hands, and fold it so that it is pulled.
Flush the tip of the pipette with a gas burner. If it is too hot, the tip closes, and if it is small, the egg or embryo is caught and broken.