Development of gene function analysis technology in the genus Neopyropia (stable transformation)

The gene construct utilizing a codon-modified hygromycin resistance gene (Pyaph7) as a selection marker was introduced into the Neopyropia yezoensis cells by the gene gun method. After that, the Neopyropia yezoensis cells were selected with hygromycin. As a result, the target genes (PyGUS gene and AmCFP gene) were stably expressed.

*Blue-stained algae are those in which PyGUS and AmCFP are expressed.

 Gene constructs introduced into Neopyropia yezoensis cells

Description of the introduced gene constructs

PyElf1 pro:
A promoter of the Elongation factor 1 gene from Neopyropia yezoensis

Pyaph7:
Codon modified hygromycin resistance gene

PyAct1 pro:
A promoter of the actin gene from Neopyropia yezoensis

PyGUS:
Codon-modified GUS (β-glucuronidase) gene

PtHSP pro:
A promoter of the HSP gene from Neopyropia tenera

AmCFP:
Cyan fluorescent protein from sea anemones