Section outline

    • The objective was to develop a marker gene for selecting transgenic individuals. A modified hygromycin resistance gene (Pyaph7), which was adapted to the codon of Neopyropia yezoensis, was created and introduced into Neopyropia yezoensis cells using a gene gun method. After that, the Neopyropia yezoensis cells were treated with the antibiotic hygromycin. As a result, Pyaph7 was introduced into the resulting hygromycin-resistant strain, and the transgenic individuals were stably isolated.

    • PyAct1 promoter and Pyaph7



      PyAct1 promoter: Promoter of the actin gene from Neopyropia yezoensis
      Pyaph7: Modified hygromycin (Hyg) resistance gene adapted to the codon of Neopyropia yezoensis



      selecting transgenic individuals