Garis besar topik

    • Environmental DNA analysis can be broadly used by sampling soil, sediment, water and ice, and a wide range of studies on eDNA in aquatic environments have been reported. However, most studies were conducted in rivers, lakes or coastal areas within 10 km from land and oceanic surveys with eDNA are still rare.

    • [Open Access] Yates et al., 2019, Environmental DNA URL

      　　

    • Water sampling in the offshore ocean is not easy because it requires a ship or research vessel with proper equipment (e.g., Niskin bottles). In addition, since the spatial dimension is larger and DNA is likely more diluted in the ocean, large volumes of water are necessary to detect DNA in low densities in the water column. Bessey et al. (2020) investigated how water sample volume affects the detections of species in a fish community by using various water sample volumes from 25 to 2000 ml, and more species were detected as more water was filtered.

    • [Open Access] Bessey et al., 2020, Environmental DNA URL

      　

    • Here I introduce the methods of water sampling and filtration for eDNA collection in ship.