Figs.
(left) Samples from the ocean (environmental microorganisms), rightward arrow: metagenomic extraction
(middle) Metagenomic DNA, upper right arrow: method 1) Incorporating DNA into culturable bacteria, lower left arrow: method 2) Sequencing all DNA
There are two major methods to find useful genes from the acquired metagenomic DNA.
One is to randomly incorporate them into easily culturable bacteria (e.g., E. coli) and look for functional genes in them (1) random cloning), and the other is to determine the sequence of all DNA fragments and look for functional genes in the DNA information (2) full sequencing).
