Figs.
(left) Algaecides and genomic DNA
(middle) Full-length genome
(right) Biosynthetic gene analysis using bioinformatics tools
The first step in revealing the biosynthetic genes is to determine the genome of the algaecide
bacteria. This requires next-generation sequencing, a high-specification instrument for DNA sequencing, but with current technology, determining bacterial genomes is not difficult (although it is expensive).
Once the full-length genome is determined, we can use various bioinformatics tools to find gene clusters for biosynthesis of compounds. The figure above is the result of using the antiSMASH tool, which shows us the gene cluster composition and the structure
of the expected product.
