Section outline

    • We are also working on the development of gene transfer technology, which is an important technology as one of the molecular biology methods. For stable expression of the gene introduced in the cells of Neopyropia yezoensis, it is important to use a promoter derived from Neopyropia yezoensis gene, a region that controls gene expression, and to modify the DNA sequence of the transgene to match Neopyropia yezoensis (codon optimization). Currently, we are applying this technology to develop a genome editing technology that can easily destroy the target gene.


    •  

      The gene construct above using the codon-modified hygromycin resistance gene (Pyaph7) as a selection marker was introduced into Neopyropia yezoensis cells by the particle gun method. Then, by selecting with an antibiotics hygromycin, the target gene (such as PyGUS gene) can be stably expressed. The blue-stained algae are those expressing the PyGUS gene, while the red ones are not.