セクションアウトライン

    • Eels complete egg maturation and ovulation approximately 18 hours after the administration of DHP and become ready for spawning.

      For fertilization, sperms taken from male were added to the eggs taken from female, and then  placed in seawater for fertilization.


    • 　Collection of unfertilized eggs from eels

    • Fertilized eggs in good condition are empirically known to float.

      Sunken eggs are not used for the subsequent observations.

      The floating rate can be calculated by counting the number of floating eggs out of all the eggs.

      Floating rate (%) = number of floating eggs / number of artificially inseminated eggs x 100