Research Example (1)

The first step o developing an immunochemical detection and measurement method is to prepare a highly pure protein of interest as an "immune antigen." The process of increasing the purity of a target protein from a sample containing multiple proteins is called "purification."

Example of purification of oogenesis related protein:

The yolk protein precursor is called vitellogenin (vitellogenin: Vg). The figure on the left is a flowchart of the purification of three types of Vg from the blood of a mullet (Mugil cephalus) to which female hormones have been administered. For example, in the case of VgA, we first use one POROS HQ of the ion-exchange column, followed by a negative affinity column combining the hydroxyapatite column and VgB acid, and finally one Superose 6 of the gel filtration column, which is purified through a total of four-column chromatographies.

Example of the chromatogram of gel filtration column:

Gel filtration column is a method to separate based on the differences in the molecules of protein. The figure on the left is a VgA elution chromatogram with the vertical axis indicating the absorbance value and the horizontal axis indicating the elution fraction number. Generally speaking, if you get one symmetrical peak, we think it is highly purified.